Shoot tip culture as a method of micropropagation of Hevea

Abstract

Shoot tips of Hevea from aseptically grown seeedlings were established in culture on Murashige and Skoog (MS) liquid medium with half strenth salts, supplimented with BAP +IBA or MS solid medium, with full strenth salts with and without BAP + IBA. BAP was found to be better for axillary bud proliferation and growth than the other cytokinines. Proliferation of buds improved with successive subcultures. A multiplication rate of 30 + 2 shoots per explant was obtained with threee subcultures in 165 days