Browsing by Author "Gunatilleke, I.D."
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Item Shoot tip culture as a method of micropropagation of Hevea(Rubber Research Institute. Agalawatta, 1988) Gunatilleke, I.D.; Samaranayake, C.Shoot tips of Hevea from aseptically grown seeedlings were established in culture on Murashige and Skoog (MS) liquid medium with half strenth salts, supplimented with BAP +IBA or MS solid medium, with full strenth salts with and without BAP + IBA. BAP was found to be better for axillary bud proliferation and growth than the other cytokinines. Proliferation of buds improved with successive subcultures. A multiplication rate of 30 + 2 shoots per explant was obtained with threee subcultures in 165 daysItem Shoot tip culture as a method of micropropagation of Hevea(Rubber Research Institute:Agalawatta, 1988) Gunatilleke, I.D.; Samaranayake, C.Shoot tips of Hevea from aseptically grown seeedlings were established in culture on Murashige and Skoog (MS) liquid medium with half strenth salts, supplimented with BAP +IBA or MS solid medium, with full strenth salts with and without BAP + IBA. BAP was found to be better for axillary bud proliferation and growth than the other cytokinines. Proliferation of buds improved with successive subcultures. A multiplication rate of 30 + 2 shoots per explant was obtained with threee subcultures in 165 days